total fgf 21 serum levels  (BioVendor Instruments)

Journal: Science Advances

Article Title: Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4

doi: 10.1126/sciadv.abo0340

Figure Lengend Snippet: ( A ) Electron micrographs of soleus muscle showing intermyofibrillar mitochondria in sections from indicated mice fed CD or HFD. Scale bars, 200 nm. n = 3 to 4. ( B and C ) Mitochondrial respiration rates were determined on mitochondria isolated from muscles of indicated mice using pyruvate (B) or succinate (C) as substrates. Pyruvate/malate (Py/M)- or Suc/Rot-stimulated respiration is shown. n = 3 to 7. ( D ) Heatmap analysis of genes differentially regulated in LONP1 mKO muscles. Each group is represented by RNA sequencing (RNA-seq) data from two independent samples generated from muscles of 2- and 6-week-old mice. Red, relative increase in abundance; blue, relative decrease. ( E ) Gene Ontology (GO) enrichment analysis of genes that were induced early at 2 weeks old and were further enhanced by LONP1 abrogation at 6 weeks old, with top 10 terms shown. The dot size reflects the gene count. ( F ) Expression of genes (qRT-PCR) related to UPR, amino acid metabolism, one-carbon metabolism, and myokines in muscles from HFD-fed LONP1 mKO mice. n = 6. ( G ) Serum FGF21 and GDF15 levels. n = 6 to 9. Color legend for the panel: white, WT CD; gray, LONP1 mKO CD; orange, WT HFD; diagonal hatch, LONP1 mKO HFD. Values represent means ± SEM. * P < 0.05 versus corresponding WT controls. Two-tailed unpaired Student’s t test (B, C, and F) or one-way ANOVA (G) was performed.

Article Snippet: Nonfasting serum FGF21 levels were measured by an FGF21 ELISA kit (Proteintech, KE10042) according to the manufacturer’s instructions.

Techniques: Isolation, Muscles, RNA Sequencing, Generated, Expressing, Quantitative RT-PCR, Two Tailed Test

Journal: Obesity Science & Practice

Article Title: Increased fructose consumption has sex‐specific effects on fibroblast growth factor 21 levels in humans

doi: 10.1002/osp4.360

Figure Lengend Snippet: Metabolic parameters for the intervention ( n = 20) and control ( n = 19) groups before (week 0) and after (week 2) the study period

Article Snippet: Intact serum FGF21 levels were measured using the Human Intact FGF21 ELISA kit from Eagle Biosciences.

Techniques: Control

Journal: Obesity Science & Practice

Article Title: Increased fructose consumption has sex‐specific effects on fibroblast growth factor 21 levels in humans

doi: 10.1002/osp4.360

Figure Lengend Snippet: (A) Baseline fasting fibroblast growth factor 21 (FGF21) levels before and after the study period for men and women. Women had significantly higher baseline FGF21 levels and decreased baseline FGF21 levels after 2 weeks of fructose exposure ( p = 0.04). (B) Fructose‐stimulated FGF21 levels before and after the study period for men and women. Women had significantly higher FGF21 levels than men at both time points ( * p < 0.008). (C) The fold change of FGF21 following 75 g of oral fructose tolerance test (OFTT) was significantly elevated in women after fructose intervention ( * p < 0.05; ** p < 0.01).

Article Snippet: Intact serum FGF21 levels were measured using the Human Intact FGF21 ELISA kit from Eagle Biosciences.

Techniques:

Journal: Obesity Science & Practice

Article Title: Increased fructose consumption has sex‐specific effects on fibroblast growth factor 21 levels in humans

doi: 10.1002/osp4.360

Figure Lengend Snippet: Sex differences in the effects of high‐fructose exposure (men: n = 8; women: n = 11)

Article Snippet: Intact serum FGF21 levels were measured using the Human Intact FGF21 ELISA kit from Eagle Biosciences.

Techniques:

Journal: Obesity science & practice

Article Title: Fibroblast growth factor 21 and fructose dynamics in humans.

doi: 10.1002/osp4.295

Figure Lengend Snippet: Figure 1 The serum FGF21 response to various doses of fructose consumption in healthy adults. (A) Average serum FGF21 response to consumption of 10 g fructose. (B) Average serum FGF21 response to consumption of 20 g fructose. (C) Average serum FGF21 response to consumption of 30 g fructose. (D) Average serum FGF21 response to consumption of 50 g fructose. (E) Average serum FGF21 response to consumption of 75 g fructose. *p < 0.05. FGF21, fibroblast growth factor 21

Article Snippet: Biochemical analysis Serum total FGF21 levels were measured using a commercially available ELISA assay (R&D Systems, Inc., MN, USA), and serum intact FGF21 levels were measured using an intact FGF21 ELISA kit (Eagle Biosciences, Nashua, NH, USA).

Techniques: